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cytokeratin 18 neoepitope (m30  (Enzo Biochem)
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Enzo Biochem cytokeratin 18 neoepitope (m30
Ectopic expression of C19MC attenuated the migratory capacity of HTR-8/SVneo cells without affecting cell proliferation or apoptosis. A, Photos illustrating the scratch wound healing process for HTR-8-C19 cells and HTR-8-WT cells at 0, 24, and 48 h after scratch, performed as described in Methods. Shown are representatives of three independent experiments. See also live cell imaging in Supplemental Figure 3. B, Quantification of the data in Figure 2A, showing the relative wound width *, P < .01. C, Photos illustrating the trans-well migration assay for HTR-8-C19 cells and HTR-8-WT cells. The photos were taken at 50× (upper) and 200× (lower) magnification. Shown are representatives of three independent experiments. D, Quantification of the transwell migration assay, showing the relative cell number (*, P < .01). E, Flow cytometry results indicating BrdU incorporation for HTR-8-C19 cells and HTR-8-WT cells. Shown is a typical flow cytometry graph, representing three independent experiments that were quantified in the diagram (P =NS, paired t test). F, Western immunoblot of PARP and <t>cytokeratin-18</t> expression in cell lysate from HTR-8-C19 or HTR-8-WT cells. Actin was used as a loading control. Shown is a representative blot of three independent experiments.
Cytokeratin 18 Neoepitope (M30, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Ectopic expression of C19MC attenuated the migratory capacity of HTR-8/SVneo cells without affecting cell proliferation or apoptosis. A, Photos illustrating the scratch wound healing process for HTR-8-C19 cells and HTR-8-WT cells at 0, 24, and 48 h after scratch, performed as described in Methods. Shown are representatives of three independent experiments. See also live cell imaging in Supplemental Figure 3. B, Quantification of the data in Figure 2A, showing the relative wound width *, P < .01. C, Photos illustrating the trans-well migration assay for HTR-8-C19 cells and HTR-8-WT cells. The photos were taken at 50× (upper) and 200× (lower) magnification. Shown are representatives of three independent experiments. D, Quantification of the transwell migration assay, showing the relative cell number (*, P < .01). E, Flow cytometry results indicating BrdU incorporation for HTR-8-C19 cells and HTR-8-WT cells. Shown is a typical flow cytometry graph, representing three independent experiments that were quantified in the diagram (P =NS, paired t test). F, Western immunoblot of PARP and cytokeratin-18 expression in cell lysate from HTR-8-C19 or HTR-8-WT cells. Actin was used as a loading control. Shown is a representative blot of three independent experiments.

Journal: Endocrinology

Article Title: C19MC MicroRNAs Regulate the Migration of Human Trophoblasts

doi: 10.1210/en.2014-1501

Figure Lengend Snippet: Ectopic expression of C19MC attenuated the migratory capacity of HTR-8/SVneo cells without affecting cell proliferation or apoptosis. A, Photos illustrating the scratch wound healing process for HTR-8-C19 cells and HTR-8-WT cells at 0, 24, and 48 h after scratch, performed as described in Methods. Shown are representatives of three independent experiments. See also live cell imaging in Supplemental Figure 3. B, Quantification of the data in Figure 2A, showing the relative wound width *, P < .01. C, Photos illustrating the trans-well migration assay for HTR-8-C19 cells and HTR-8-WT cells. The photos were taken at 50× (upper) and 200× (lower) magnification. Shown are representatives of three independent experiments. D, Quantification of the transwell migration assay, showing the relative cell number (*, P < .01). E, Flow cytometry results indicating BrdU incorporation for HTR-8-C19 cells and HTR-8-WT cells. Shown is a typical flow cytometry graph, representing three independent experiments that were quantified in the diagram (P =NS, paired t test). F, Western immunoblot of PARP and cytokeratin-18 expression in cell lysate from HTR-8-C19 or HTR-8-WT cells. Actin was used as a loading control. Shown is a representative blot of three independent experiments.

Article Snippet: The membranes were blocked and incubated with primary antibodies against cleaved poly (ADP-ribose) polymerase (PARP) (dilution 1:1000, Cell Signaling), cytokeratin 18 neoepitope (M30, dilution 1:1000, Enzo Life Sciences), or actin (0.08 μg/mL final concentration, Millipore).

Techniques: Expressing, Live Cell Imaging, Migration, Transwell Migration Assay, Flow Cytometry, BrdU Incorporation Assay, Western Blot